IgG subclass response to human parvovirus B19 infection
Identifieur interne : 001617 ( Istex/Checkpoint ); précédent : 001616; suivant : 001618IgG subclass response to human parvovirus B19 infection
Auteurs : Rauli Franssila [Finlande] ; Maria Söderlund [Finlande] ; Caroline S. Brown [Pays-Bas] ; Willy J. M Spaan [Pays-Bas] ; Ilkka Sepp L [Finlande] ; Klaus Hedman [Finlande]Source :
- Clinical and Diagnostic Virology [ 0928-0197 ] ; 1996.
English descriptors
- Teeft :
- Acute infection, Acute phase, Background levels, Capsid, Capsid antigen, Capsid proteins, Cell clones, Chronic infection, Convalescence, Diagnostic test, Diagnostic virology, Diphteria toxin, Early convalescence, Epitope, Erythema infectiosum, Flvp1, Flvp1 antigen, Franssila, Haartman institute, Haemophilus influenzae type, High levels, Human parvovirus, Human parvovirus infection, Hydrops fetalis, Igg1, Igg3, Igg4, Igg4 responses, Immunol, Infection, Kurtzman, Late convalescence, Linear epitopes, Monoclonal antibodies, Optimum volume, Other hand, Parvovirus, Past immunity, Primary structure, Recent infection, S6derlund, Serum pool, Serum pools, Subclass, Subclass response, Subclasses igg1, Subclasses igg3, Time point, Time points, Total igg4, Unique portion, Virology, Years days.
Abstract
Abstract: Background: IgG antibodies are essential to immunity against human parvovirus B19 and can neutralize infection both in bone marrow cell cultures infected in vitro and in chronically infected immunosuppressed individuals. Objectives: To assess the levels and response kinetics of IgG subclasses towards individual structural proteins of human parvovirus B19. Study design: Subclasses of IgG for capsid proteins VP1 or VP2 were quantified by EIA using monoclonal antibodies in 30 acutely infected and 30 convalescent patients, as well as in 32 remotely infected and 20 non-infected controls. Results: In all groups of seropositive individuals the predominant subclass for either structural protein was IgG1. Subclass IgG3 was associated with acute infection. By contrast, IgG4 appeared months after infection, and occurred specifically towards VP1. The ratio of VP1-specific subclasses IgG3 and IgG4 provided a diagnostic test for recent infection with a specificity of 98% and a sensitivity of 97%. Conclusions: Comparative measurement of VP1-specific IgG3 and IgG4 is useful in diagnosis. The IgG4 results point to long-term expression of immunologically active VP1 and to T-cell help of Th2 type for B-cells recognizing VP1.
Url:
DOI: 10.1016/0928-0197(96)00156-0
Affiliations:
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M Spaan</name></author><author><name sortKey="Sepp L, Ilkka" sort="Sepp L, Ilkka" uniqKey="Sepp L I" first="Ilkka" last="Sepp L">Ilkka Sepp L</name></author><author><name sortKey="Hedman, Klaus" sort="Hedman, Klaus" uniqKey="Hedman K" first="Klaus" last="Hedman">Klaus Hedman</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A2A05B052207B90FB3BC3847436BAB6B9C1BEB0F</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1016/0928-0197(96)00156-0</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-B9VMBL4H-W/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001614</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001614</idno><idno type="wicri:Area/Istex/Curation">001614</idno><idno type="wicri:Area/Istex/Checkpoint">001617</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001617</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">IgG subclass response to human parvovirus B19 infection</title><author><name sortKey="Franssila, Rauli" sort="Franssila, Rauli" uniqKey="Franssila R" first="Rauli" last="Franssila">Rauli Franssila</name><affiliation wicri:level="4"><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Virology, Haartman Institute, P.O. Box 21, FIN-00014, University of Helsinki, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation></author><author><name sortKey="Soderlund, Maria" sort="Soderlund, Maria" uniqKey="Soderlund M" first="Maria" last="Söderlund">Maria Söderlund</name><affiliation wicri:level="4"><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Virology, Haartman Institute, P.O. Box 21, FIN-00014, University of Helsinki, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation></author><author><name sortKey="Brown, Caroline S" sort="Brown, Caroline S" uniqKey="Brown C" first="Caroline S" last="Brown">Caroline S. Brown</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Virology, University Hospital of Leiden, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Spaan, Willy J M" sort="Spaan, Willy J M" uniqKey="Spaan W" first="Willy J. M" last="Spaan">Willy J. M Spaan</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Virology, University Hospital of Leiden, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Sepp L, Ilkka" sort="Sepp L, Ilkka" uniqKey="Sepp L I" first="Ilkka" last="Sepp L">Ilkka Sepp L</name><affiliation wicri:level="4"><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation></author><author><name sortKey="Hedman, Klaus" sort="Hedman, Klaus" uniqKey="Hedman K" first="Klaus" last="Hedman">Klaus Hedman</name><affiliation wicri:level="4"><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Virology, Haartman Institute, P.O. Box 21, FIN-00014, University of Helsinki, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Clinical and Diagnostic Virology</title><title level="j" type="abbrev">DIAVIR</title><idno type="ISSN">0928-0197</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">6</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="41">41</biblScope><biblScope unit="page" to="49">49</biblScope></imprint><idno type="ISSN">0928-0197</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0928-0197</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acute infection</term><term>Acute phase</term><term>Background levels</term><term>Capsid</term><term>Capsid antigen</term><term>Capsid proteins</term><term>Cell clones</term><term>Chronic infection</term><term>Convalescence</term><term>Diagnostic test</term><term>Diagnostic virology</term><term>Diphteria toxin</term><term>Early convalescence</term><term>Epitope</term><term>Erythema infectiosum</term><term>Flvp1</term><term>Flvp1 antigen</term><term>Franssila</term><term>Haartman institute</term><term>Haemophilus influenzae type</term><term>High levels</term><term>Human parvovirus</term><term>Human parvovirus infection</term><term>Hydrops fetalis</term><term>Igg1</term><term>Igg3</term><term>Igg4</term><term>Igg4 responses</term><term>Immunol</term><term>Infection</term><term>Kurtzman</term><term>Late convalescence</term><term>Linear epitopes</term><term>Monoclonal antibodies</term><term>Optimum volume</term><term>Other hand</term><term>Parvovirus</term><term>Past immunity</term><term>Primary structure</term><term>Recent infection</term><term>S6derlund</term><term>Serum pool</term><term>Serum pools</term><term>Subclass</term><term>Subclass response</term><term>Subclasses igg1</term><term>Subclasses igg3</term><term>Time point</term><term>Time points</term><term>Total igg4</term><term>Unique portion</term><term>Virology</term><term>Years days</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Background: IgG antibodies are essential to immunity against human parvovirus B19 and can neutralize infection both in bone marrow cell cultures infected in vitro and in chronically infected immunosuppressed individuals. Objectives: To assess the levels and response kinetics of IgG subclasses towards individual structural proteins of human parvovirus B19. Study design: Subclasses of IgG for capsid proteins VP1 or VP2 were quantified by EIA using monoclonal antibodies in 30 acutely infected and 30 convalescent patients, as well as in 32 remotely infected and 20 non-infected controls. Results: In all groups of seropositive individuals the predominant subclass for either structural protein was IgG1. Subclass IgG3 was associated with acute infection. By contrast, IgG4 appeared months after infection, and occurred specifically towards VP1. The ratio of VP1-specific subclasses IgG3 and IgG4 provided a diagnostic test for recent infection with a specificity of 98% and a sensitivity of 97%. Conclusions: Comparative measurement of VP1-specific IgG3 and IgG4 is useful in diagnosis. The IgG4 results point to long-term expression of immunologically active VP1 and to T-cell help of Th2 type for B-cells recognizing VP1.</div></front></TEI><affiliations><list><country><li>Finlande</li><li>Pays-Bas</li></country><region><li>Hollande-Méridionale</li><li>Uusimaa</li></region><settlement><li>Helsinki</li><li>Leyde</li></settlement><orgName><li>Université d'Helsinki</li></orgName></list><tree><country name="Finlande"><region name="Uusimaa"><name sortKey="Franssila, Rauli" sort="Franssila, Rauli" uniqKey="Franssila R" first="Rauli" last="Franssila">Rauli Franssila</name></region><name sortKey="Hedman, Klaus" sort="Hedman, Klaus" uniqKey="Hedman K" first="Klaus" last="Hedman">Klaus Hedman</name><name sortKey="Sepp L, Ilkka" sort="Sepp L, Ilkka" uniqKey="Sepp L I" first="Ilkka" last="Sepp L">Ilkka Sepp L</name><name sortKey="Soderlund, Maria" sort="Soderlund, Maria" uniqKey="Soderlund M" first="Maria" last="Söderlund">Maria Söderlund</name></country><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="Brown, Caroline S" sort="Brown, Caroline S" uniqKey="Brown C" first="Caroline S" last="Brown">Caroline S. Brown</name></region><name sortKey="Spaan, Willy J M" sort="Spaan, Willy J M" uniqKey="Spaan W" first="Willy J. M" last="Spaan">Willy J. M Spaan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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